glcK [2017-8-11 16:0:3]

glucose kinase (D-glucose:ATP)

locus: BSU24850

pI: 5.99

mw: 33.39 kDa

protein length: 321 aa

gene length: 963 bp

function: phosphorylation of the free glucose moiety resulting from cleavage of di-and oligosaccharides

product: glucose kinase (D-glucose:ATP)

essential: no

ec: 2.7.1.2

synonyms: yqgR

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.20|Utilization of trehalose]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.22|Utilization of maltose]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.23|Utilization of starch/ maltodextrin]

Gene

Coordinates: 2,570,606 → 2,571,571

The protein

Catalyzed reaction/ biological activity

• ATP + D-glucose = ADP + D-glucose 6-phosphate (according to Swiss-Prot)

Protein family

• ROK (nagC/xylR) family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|AF4395D134485290F4AA307B48494FED39E52CD7|XylR]

Structure

• [PDB|2QM1] (from ''Enterococcus faecalis'', 38% identity, 52% similarity)

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|89C51789FBB2EE86771ABD8629FD99748ED5D4EF|yqgP]-[gene|C44F6723A2F1A627A0B9042871A07F5365D6121A|yqgO]-[gene|8E10A8B566BC25CBDDEF1503656A4234FD29ACB8|glcK]-[gene|2B33F2F9C2FB0767B72A29D060037393C88ACEE5|yqgS]
  description: [pubmed|22383849]

  additional information

  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]∼P binds to the promoter region, but regulation has not been explored [pubmed|25666134]

Biological materials

Mutant

• GP2 (spc), available in the [SW|Stülke] lab

References

• 9620975,15050034,15018644,16707683,7952186,27324299